Abstract Carbohydrates serve as a primary energy source and structural components in biological systems. The identification of carbohydrates in unknown samples is a fundamental procedure in biochemistry. The Molisch test is the standard general test for the detection of carbohydrates. This article discusses the principle, procedure, and application of the Molisch test, highlighting a modified ("patched") procedure designed to enhance safety, efficiency, and color differentiation in the detection of trace carbohydrate quantities.
) menghidrolisis ikatan glikosidik dan mendehidrasi gula menjadi (untuk pentosa) atau 5-hidroksimetil furfural (untuk heksosa). Kondensasi : Senyawa furfural tersebut bereaksi dengan reagen Molisch -naftol yang dilarutkan dalam etanol). Pembentukan Kompleks : Hasil reaksi membentuk cincin berwarna ungu jurnal+uji+molisch+karbohidrat+patched
Metode patched menunjukkan akurasi 94% dibandingkan KLT dalam studi pada 50 sampel pangan (Jurnal Pangan dan Gizi, Vol 12, 2022). Fessenden, R
The Molisch test is significant for several reasons: Dropdown to select carbohydrate sample (glucose
Visualization: A successful reaction is marked by the appearance of a purple or reddish-purple ring at the junction between the acid and the test solution. 3. Methodology Standard procedures involve the following steps:
Uji Molisch dengan metode patch test dapat digunakan dalam berbagai bidang, yaitu: